Microbiology experiments : a health science perspective / John Kleyn, Mary Bicknell, Anna Oller.

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Bibliographic Details
Main Author: Kleyn, John G.
Other Authors: Bicknell, Mary., Oller, Anna.
Format: Book
Published: New York : McGraw Hill, ©2012.
Edition:7th ed.
Table of Contents:
  • pt. 1. Basic microbiology. Introduction to microbiology. Ubiquity of microorganisms ; Pure culture and aseptic technique ; Introduction to the compound light microscope ; The oil immersion lens
  • Introduction to staining of microorganisms. Simple stains: positive and negative stains ; Differential and other special stains
  • Introduction to microbial growth. Chemically defined, complex, selective, and differential media ; Quantification of microorganisms
  • Introduction to the environment and microbial growth. Aerobic and anaerobic growth ; The effect of incubation temperature on generation time
  • Introduction to control of microbial growth. Moist and dry heat sterilization: thermal death point and thermal death time ; Control of microbial growth with ultraviolet light ; Changes in osmotic pressure due to salt and sugar and their effects on microbial growth ; Antibiotics ; Antiseptics and disinfectants
  • Introduction to microbial genetics. Selection of bacterial mutants resistant to antibiotics ; Transformation: a form of genetic recombination ; Gene regulation: induction and catabolite repression
  • pt. 2. The other microbial world. Introduction to the other microbial world. Microscopic identification of fungi ; Parasitology: protozoa and helminths ; Titering prokaryotic viruses
  • pt. 3. Microbes and humans. Introduction to medical microbiology. Normal skin biota ; Streptococci and respiratory microorganisms ; Identification of enteric gram-negative rods ; Clinical unknown identification
  • pt. 4. Immunology. Introduction to some immunological principles and techniques. Differential white blood cell stains ; Lysozyme, an enzyme contributing to innate immunity ; Enzyme-linked immunosorbent assay (ELISA)
  • pt. 5. Public health. Introduction to the prevention and control of communicable diseases. Bacteriological examination of water: multiple-tube fermentation and membrane filter techniques ; Epidemiology
  • pt. 6. Applications of biotechnology. Introduction to biotechnology. Identifying DNA with restriction enzymes and the Polymerase Chain Reaction (PCR) ; Electrophoresis ; Identification of bacteria using the ribosomal database project ; Microarray
  • pt. 7. Individual projects. Introduction to the individual projects. Hydrocarbon-degrading bacteria: cleaning up after oil spills ; Luminescent bacteria: bacteria that produce light
  • Appendices. Culture media and reagent formulas ; Living microorganisms (bacteria, fungi, protozoa, and helminths) chosen for study in this manual ; Dilution practice problems ; Alternative procedures ; Use of the ocular micrometer for measurement of relative and absolute cell size ; History and working principles of light microscopy.