Microbiology experiments : a health science perspective / John Kleyn, Mary Bicknell, Anna Oller.
New York :
Table of Contents:
- pt. 1. Basic microbiology. Introduction to microbiology. Ubiquity of microorganisms ; Pure culture and aseptic technique ; Introduction to the compound light microscope ; The oil immersion lens
- Introduction to staining of microorganisms. Simple stains: positive and negative stains ; Differential and other special stains
- Introduction to microbial growth. Chemically defined, complex, selective, and differential media ; Quantification of microorganisms
- Introduction to the environment and microbial growth. Aerobic and anaerobic growth ; The effect of incubation temperature on generation time
- Introduction to control of microbial growth. Moist and dry heat sterilization: thermal death point and thermal death time ; Control of microbial growth with ultraviolet light ; Changes in osmotic pressure due to salt and sugar and their effects on microbial growth ; Antibiotics ; Antiseptics and disinfectants
- Introduction to microbial genetics. Selection of bacterial mutants resistant to antibiotics ; Transformation: a form of genetic recombination ; Gene regulation: induction and catabolite repression
- pt. 2. The other microbial world. Introduction to the other microbial world. Microscopic identification of fungi ; Parasitology: protozoa and helminths ; Titering prokaryotic viruses
- pt. 3. Microbes and humans. Introduction to medical microbiology. Normal skin biota ; Streptococci and respiratory microorganisms ; Identification of enteric gram-negative rods ; Clinical unknown identification
- pt. 4. Immunology. Introduction to some immunological principles and techniques. Differential white blood cell stains ; Lysozyme, an enzyme contributing to innate immunity ; Enzyme-linked immunosorbent assay (ELISA)
- pt. 5. Public health. Introduction to the prevention and control of communicable diseases. Bacteriological examination of water: multiple-tube fermentation and membrane filter techniques ; Epidemiology
- pt. 6. Applications of biotechnology. Introduction to biotechnology. Identifying DNA with restriction enzymes and the Polymerase Chain Reaction (PCR) ; Electrophoresis ; Identification of bacteria using the ribosomal database project ; Microarray
- pt. 7. Individual projects. Introduction to the individual projects. Hydrocarbon-degrading bacteria: cleaning up after oil spills ; Luminescent bacteria: bacteria that produce light
- Appendices. Culture media and reagent formulas ; Living microorganisms (bacteria, fungi, protozoa, and helminths) chosen for study in this manual ; Dilution practice problems ; Alternative procedures ; Use of the ocular micrometer for measurement of relative and absolute cell size ; History and working principles of light microscopy.