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|b .K54 2012
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| 100 |
1 |
|
|a Kleyn, John G.
|0 n 78004724
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| 245 |
1 |
0 |
|a Microbiology experiments :
|b a health science perspective /
|c John Kleyn, Mary Bicknell, Anna Oller.
|
| 250 |
|
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|a Seventh edition.
|
| 264 |
|
1 |
|a New York :
|b McGraw Hill,
|c [2012]
|
| 264 |
|
4 |
|c ©2012
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| 300 |
|
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|a viii, 310 pages, 14 unnumbered pages of plates :
|b illustrations (some color) ;
|c 28 cm
|
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|a text
|b txt
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|a Includes bibliographical references and index.
|
| 505 |
0 |
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|a pt. 1. Basic microbiology. Introduction to microbiology. Ubiquity of microorganisms ; Pure culture and aseptic technique ; Introduction to the compound light microscope ; The oil immersion lens -- Introduction to staining of microorganisms. Simple stains: positive and negative stains ; Differential and other special stains -- Introduction to microbial growth. Chemically defined, complex, selective, and differential media ; Quantification of microorganisms -- Introduction to the environment and microbial growth. Aerobic and anaerobic growth ; The effect of incubation temperature on generation time -- Introduction to control of microbial growth. Moist and dry heat sterilization: thermal death point and thermal death time ; Control of microbial growth with ultraviolet light ; Changes in osmotic pressure due to salt and sugar and their effects on microbial growth ; Antibiotics ; Antiseptics and disinfectants -- Introduction to microbial genetics. Selection of bacterial mutants resistant to antibiotics ; Transformation: a form of genetic recombination ; Gene regulation: induction and catabolite repression -- pt. 2. The other microbial world. Introduction to the other microbial world. Microscopic identification of fungi ; Parasitology: protozoa and helminths ; Titering prokaryotic viruses -- pt. 3. Microbes and humans. Introduction to medical microbiology. Normal skin biota ; Streptococci and respiratory microorganisms ; Identification of enteric gram-negative rods ; Clinical unknown identification -- pt. 4. Immunology. Introduction to some immunological principles and techniques. Differential white blood cell stains ; Lysozyme, an enzyme contributing to innate immunity ; Enzyme-linked immunosorbent assay (ELISA) -- pt. 5. Public health. Introduction to the prevention and control of communicable diseases. Bacteriological examination of water: multiple-tube fermentation and membrane filter techniques ; Epidemiology -- pt. 6. Applications of biotechnology. Introduction to biotechnology. Identifying DNA with restriction enzymes and the Polymerase Chain Reaction (PCR) ; Electrophoresis ; Identification of bacteria using the ribosomal database project ; Microarray -- pt. 7. Individual projects. Introduction to the individual projects. Hydrocarbon-degrading bacteria: cleaning up after oil spills ; Luminescent bacteria: bacteria that produce light -- Appendices. Culture media and reagent formulas ; Living microorganisms (bacteria, fungi, protozoa, and helminths) chosen for study in this manual ; Dilution practice problems ; Alternative procedures ; Use of the ocular micrometer for measurement of relative and absolute cell size ; History and working principles of light microscopy.
|
| 650 |
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|a Microbiology
|v Laboratory manuals.
|0 sh2008107741
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| 650 |
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|a Microbiology
|x Experiments.
|0 sh 85084786
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| 650 |
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|a Microorganisms
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|0 sh 85084725
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| 700 |
1 |
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|a Bicknell, Mary.
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|a Oller, Anna.
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